Translational Research Lab

Instrument Applications

Accordion Content

ASSIST PLUS Automated Pipet Robot

Manufacturer: INTEGRA
Location: PSC 510
Description/Applications: Electronic multi-channel pipettes (12.5 μL, 300 μL, and 1250 μL) along with automated liquid handling robot streamline pipetting to ensure superior accuracy, reproducibility without error. Pipetting modes include mixing, dilution, repeat or variable dispense, and multi aspirate for 96- or 384-well plates.
Supporting Documents: Instrument Manual

 

KingFisher Duo Prime

Manufacturer: ThermoFisher
Location: PSC 402E
Description/Applications: Automated purification system for DNA/RNA/protein from a variety of starting materials provides high yield, reproducibility and minimal hand-on time. Auto-decontamination with UV prevents cross-contamination. MagMix from Thermo Scientific™ or other compatible magnetic beads are applicable.
Supporting Documents: Instrument Manual

 

Prospect Edge 200 Separation System

Manufacturer: Prospect Biosystems
Location: PSC 402B
Description/Applications: Non-denaturing, front-end sample fractionation using extracting media of different densities.
Supporting Documents: Instrument Manual

 

Qiagen TissueLyser II

Manufacturer: Qiagen
Location: PSC 402E
Description/Applications: TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads.
Supporting Documents: Instrument Manual

 

Mixer HC

Manufacturer: USA Scientific
Location: PSC 402E
Description/Applications: Mixer HC is for heating, cooling, and mixing almost any of your lab vessels. Improve assay results by dry incubation and mixing of your samples simultaneously.
Supporting Documents:

Bio-Plex Suspension Luminex Array

Manufacturer: Bio-Rad
Location: PSC 510
Description/Applications: Both magnetic and polystyrene-bead based Luminex multiplex analysis of up to 100 biomolecules (proteins, peptides or nucleic acids) in a single sample.
Supporting Documents: Instrument Manual

 

Countess II FL Automated Cell Counter

Manufacturer: ThermoFisher
Location: PSC 510
Description/Applications: Quickly and easily counts cells, monitors fluorescent protein expression, and measures cell viability.
Supporting Documents: Instrument Manual

 

Meso Scale Discovery (MSD) Sector Imager 2400

Manufacturer: ThermoFisher
Location: PSC 402E
Description/Applications: MSD features excellent sensitivity and dynamic range for multiplex assay capability using 96- well plates and reads single-assay, 384-well plates, while providing speed suitable for medium throughput laboratories.
Supporting Documents: Instrument Manual

 

Shandon Cytospin 4

cytospinManufacturer: ThermoFisher
Location: PSC 402E
Description/Applications: Provides economical thin-layer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine.
Supporting Documents: Cytospin 4 Manual

 

Seahorse XFe96 Analyzer

Manufacturer: Agilent
Location: PSC 402E
Description/Applications: XFe96 (96-well format) measures mitochondrial respiration and glycolysis to characterize cellular metabolic function in cultures cells and ex-vivo samples.
Supporting Documents: Instrument Manual

 

Seahorse XFp Analyzer

Manufacturer: Agilent
Location: PSC 402E
Description/Applications: XFp (8-well format) measures mitochondrial respiration and glycolysis to characterize cellular metabolic function in cultures cells and ex-vivo samples.
Supporting Documents: Instrument Manual

FACSAria Fusion Cell Sorter

Manufacturer:BD Bioscience
Location: 
PSC B6
Description/Applications:
Comprehensive flow cytometry sorter with biosafety regulation provides exceptional multi-color performance and maximal sorting purity and recovery. Furthermore, Aria Fusion is cross-instrumented with our cell analyzers, Fortessa X20 & LSM II. Current 2 lasers are: 1) 488 nm (Em 530, 585, 616, 695, 780 nm and 2) 633 nm (Em 670, 780 nm).
Sorting options:
 – 4 way (1.5 or 5 mL tubes), 2 way (15 mL tubes), single-cell ACDU (6- to 96-well plate), or index sorting.
Supporting Documents: Instrument Manual

Fortessa X-20 Cell Analyzer

Manufacturer: BD Bioscience
Location: PSC B6
Description/Applications: Flow Cytometry Analyzer for high-performance multi-color analysis up to 11 fluorochromes with 3 laser: 1) 405 nm (Em 450, 525, 610 nm), 2) 488 nm (Em 530, 575, 610, 695, 780 nm) and 3) 633 nm (Em 670, 730, 780 nm).
Supporting Documents: Instrument Manual

LSRII Cell Analyzer

Manufacturer: BD Bioscience
Location: PSC B6
Description/Applications: Flow Cytometry Analyzer can simultaneously analyze up to 8 colors with 3 lasers: 1) 355 nm (Em 450, 530 nm), 2) 488nm (Em 530, 575, 695, 780 nm), 3) 633nm (Em 660, 780 nm)
Supporting Documents: Instrument Manual

Chemidoc Touch System

Manufacturer: Bio-Rad
Location: PSC 510
Description/Applications: Gel imaging system that detects colorimetry, densitometry, chemiluminescence and fluorescence.
Supporting Documents: Instrument Manual

 

Cytation 5 Cell Imaging Multi-Mode Reader

Manufacturer: BioTek
Location: PSC 402E
Description/Applications: Cytation 5 combines automated, digital high-resolution microscopy and fast, conventional microplate detection in configurable, upgradable platform: 4X, 10X, 20X, 40X and 60X magnification in fluorescence (blue, green, red and far-red), brightfield, color-brightfield and phase contrast, multi-mode high-throughput imaging scan detection modules, temperature control, shaker and CO2 gas controller. 6- to 384-well plate, culture dish, flask, or histology slide can be used. Cytation 5 is also applicable with Seahorse for the imaging normalization.
Supporting Documents: Instrument Manual

iBright FL1000 Gel/ Cell Imager

Manufacturer: ThermoFisher
Location: PSC 510
Description/Applications: High-performance imaging system for gels, blots and plates can capture images of color-bright field, fluorescence (violet to far-red) and luminescence. The fluorescence mode can detect up to 4 colors simultaneously at UV to far-red range.
Supporting Documents: Instrument Manual

 

IVIS Lumina Series III

44-144843IVIS-Lumina-Series-III

Manufacturer:Perkin Elmer
Location:
PSC B5
Description/Applications:
Imaging of both fluorescent and bioluminescent reporters. The system is equipped with filter sets to image reporters that emit from green to near-infrared, an XGI-8 Gas Anesthesia System, and can image up to 5 mice at a time. This instrument may also be used for fluorescent imaging of gels or cell culture plates. 
Other info: 
PIs must have an approved IACUC protocol in place to use this instrument. Users must also provide their own isoflurane; an oxygen tank is provided by the core.
Supporting Documents: Instrument Manual

Zeiss Axio Scope A1

Manufacturer: ZEISS 
Location:
PSC 402D
Description/Applications: 
Fluorescent microscope that scan bright field, blue, green and red fluorescence images.
Supporting Documents: ZEISS AxioScope A1

 

 

Zeiss LSM 880 + Airyscan Confocal Microscope

Manufacturer: ZEISS 
Location:
PSC 406G
Description/Applications: 
Confocal microscope with Airyscan module (fast, super-resolution scan): 9 lasers (355, 405, 458, 488, 514, 561, 594, 633 and 690-1040 nm); up to 32 fluorescence colors; long-term live scan with temperature and CO2 controlled incubator.
Supporting Documents:
Please contact TRLab.

Epoch2 Microplate Spectrophotometer

Manufacturer: BioTek
Location: PSC 510
Description/Applications: High-performance, flexible monochromator-based multi-mode microplate reader for absorbance with new incubator technology (up to 65ºC) with condensation control allows long-term kinetic analysis for cellular and microbial growth.
Options: 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate
Supporting Documents: Instrument Information

Synergy H1 Hybrid Multi-mode Plate Reader

Manufacturer: BioTek
Location: PSC 510
Description/Applications: High-performance, flexible monochromator-based, hybrid multi-mode microplate reader for absorbance, fluorescence intensity, fluorescence polarization, time-resolved fluorescence, and luminescence-based applications. The new incubator technology (up to 65°C) with condensation control allows long-term kinetic analysis. Additional injectors allow for dynamic assays.
Options: 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate
Supporting Documents: Instrument Information

 

Hidex 300 SL Scintillation Counter

Manufacturer: Hidex
Location: PSC 603
Description/Applications: Automatic Liquid Scintillation Counter with 3 PMT detectors enable triple-to-double coincidence ratio (TDCR) counting, which is an absolute counting method for obtaining counting efficiency of the samples without external or internal standard sources. Samples sizes are 40 (20 mL) or 96 (7 mL). Uses MicroWin software to collect, analyze and export data.
Options: 6- to 384-well plate format, cuvettes and Take3™ Micro-Volume Plate
Supporting Documents: Hidex 300SL User Manaul

 

F-2500 Fluorescence Spectrophotometer

Manufacturer: Hitachi
Location: PSC 603
Description/Applications: Fluorescence monochromator-based analysis (220-730 nm); Wavelength scanning speeds up to 3000 nm/min; 2 nm resolution. Cuvette format
Supporting Documents: Instrument Manual

Jobin Yvon FluoroLog-3 Modular Spectrofluorometer

Manufacturer: Horiba
Location: PSC 510
Description/Applications: Steady-state and time-resolved characterization of fluorescent samples 300 – 600 nm. Features include single-read, kinetic-read, time-resolved scan, 3D excitation / emission scan, and temperature control. Cuvette format.
Supporting Documents: Instrument Manual

 

J-815 Circular Dichroism (CD) Spectrometer

J815CircularDichroismSpectrometerManufacturer: Jasco
Location: PSC 402B
Description/Applications: Circular dichroism (CD) is dichroism involving circularly polarized light. CD spectroscopy has a wide range of applications: characterization of a protein’s secondary structure (a-helix, ß-sheet), detection of structural changes and stability upon mutagenesis, pH ranges, temperatures, denaturants, buffers, stabilizers, etc. J-815 features peltier temperature control system, standard CD measurement as well as UV/Vis absorbance.
Supporting Documents: Instrument Usage Guide Jasco J-800 Series Users Guide

Oxford NMR AS400

Manufacturer: Oxford
Location: PSC 402G
Description/Applications: Two-channel superconducting 400 MHz NMR Spectrometer that produces very high-resolution data. Liquid and solid samples are achievable for structural analysis and identification of organic molecules.
Supporting Documents: Please contact TRLab.

 

Differential Scanning Calorimetry (DSC) DS-8500

Manufacturer: Perkin Elmer
Location: PSC 402B
Description/Applications: Thermal analysis technique used to detect transitions like melts, glass transitions, phase changes, and curing; Features double-furnace technology with fast controlled scanning rates up to 750˚C/min, which directly measures the heat flow difference between two independent furnaces.
Supporting Documents: Instrument ManualGeneral Quick Guide

 

Dynamic Light Scattering (DLS) DynaPro Plate Reader II

Manufacturer: Wyatt
Location: PSC 510
Description/Applications: Measurement of the hydrodynamic radius of particles, polymers or proteins in a solution in the size range of 2-1000 nm in diameter. Features temperature control, automated kinetics measurement settings in 96-, 384- or 1536-well plate format
Supporting Documents: User’s GuideCompatible Plates

ABI 7900HT Fast Real-Time PCR

Manufacturer: ThermoFisher
Location: PSC 510
Description/Applications: Flexible-throughput quantitative real-time PCR capabilities for target discovery through confirmation and screening. Uses 96- or 384-well plate.
Options: Microfluidic card; Sample block and sealer
Supporting Documents: Instrument Manual

 

 

 

QuantStudio K12 Flex Real-Time PCR with OpenArray Autofill Module

Acknowledgement: The acquisition of this instrument was supported by a grant from the USC Core Instrumentation Fund
Manufacturer: ThermoFisher
Location: PSC 510
Description/Applications: Performs and analyzes PCR experiments for accurate and sensitive quantification of nucleic acid targets. Uses 96- or 384-well plate, or array card
Options: Cycler; Accufill; Case sealing station
Supporting Documents: Instrument Manual

 

Gene Pulser MXcell Electroporation (96-Well Format)

Manufacturer: Bio-Rad
Location: PSC 402B
Description/Applications: Electroporation of mammalian cells , bacterial, and fungal cell types, especially useful for primary and hard-to-transfect cells
Other info: 12- or 96-well plate, or cuvette format
Supporting Documents: Instrument Manual

 

Gene Pulser Xcell Electroporation (Cuvette Format)

Manufacturer: Bio-Rad
Location: PSC 402B
Description/Applications: Electroporation of mammalian, bacterial, and fungal cell types
Other info: Cuvette format
Supporting Documents:Instrument Manual

 

NanoDrop One

Manufacturer: Thermo Scientific
Location: PSC 510
Description/Applications: Quantifies DNA, RNA and protein samples with 1-2 microliter volumes; 190-850 nm spectral range.
Charges: Pharmacy: $8/hr; Other USC: $10/hr. 15 min minimum reservation.
Supporting Documents: Instrument Manual

Qubit 3.0 Fluor0meter

Manufacturer: ThermoFisher
Location: PSC 510
Description/Applications: Portable benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity.
Supporting Documents: Instrument Manual

Agilent 4200 TapeStation

Manufacturer: Agilent
Location: PSC 510
Description/Applications: Automated microfluidic chip-based electrophoresis analysis of DNA, RNA and protein size and quantity with high-throughput scans up to 96 samples.
Other info: User needs to have reagents and assay tapes.
Supporting Documents: Instrument Manual

 

BD Rhapsody Express Single-Cell Assay

Manufacturer: BD Biosciences
Location: PSC 402E
Description/Applications: BD Rhapsody Express and its cartridge enable single-cell capture and molecular indexing of mRNA transcripts to prepare single cell mRNA sequencing libraries. Proprietary BD™ Molecular Indexing technology is used to count individual mRNA molecules.
Supporting Documents: Instrument Manual

 

Experion Automated Electrophoresis System

experionManufacturer: Bio-Rad
Location: PSC 510
Description/Applications: Automated microfluidic chip-based electrophoresis analysis of DNA, RNA and protein size and quantity
Supporting Documents: Instrument Manual

 

Illumina NGS MiSeq

Manufacturer: Illumina
Location: PSC 510
Description/Applications: MiSeq can read up to 15 Gb/run, delivering over 600 bases of sequence data per read, allowing a variety of next-generation sequencing applications, including targeted gene, small genome, and amplicon sequencing, 16S metagenomics, and more.
Supporting Documents: Instrument Manual