The Histology Laboratory

tissue samples under a microscope
The USC School of Pharmacy Histology Laboratory, located on the fourth floor of the Pharmaceutical Sciences Center, is ready to help you with your histology and pathology research. This school-wide core laboratory is open to any USC researcher and has everything you will need to make microscope slides from tissue samples. Tissues can be paraffin embedded, sectioned and stained in the laboratory. Sectioning can involve making microtome, vibratome and cryostat thin slices. You can also make color photographs of your microscopic images for publication. Expert advice is available concerning staining techniques and the interpretation of microscopic images.

Resources

The Histology Laboratory houses the following instruments and accessories:

Microm Spin Tissue Processor STP 120

Microm Spin Tissue Processor STP 120

Components/Accessories:

  • Main Unit
  • Individual Tissue Carriers

Capacity: The tissue processor automatically dehydrates and paraffin infiltrates tissue samples.

Microm Tissue Embedding Center EC 350

Microm Tissue Embedding Center EC 350

Components/Accessories:

  • Main Unit
  • Cooling Unit

Capacity: At this station, paraffin infiltrated tissues are embedded in wax and mounted on cassettes.

Microm Rotary Microtome

Microm Rotary Microtome

Components/Accessories:

  • Main Unit
  • Disposable Blades
  • Water Bath

Capacity: The microtome cuts thin sections of paraffin embedded tissue cassettes with a disposable blade. The blade can be quickly replaced as necessary. The water bath is used to float the thin sections onto microscope slides.

Vibratome

Vibratome

Components/Accessories:

  • Main Unit
  • Disposable Blades
  • Water Bath
  • Light

Capacity: The vibratome is used to cut sections of formaldehyde fixed soft tissues, such as brain. Sections are mounted onto microscope slides.

Microm Cryostat HM 525

Microm Cryostat HM 525

Capacity: Frozen tissue samples are thin sectioned with the cryostat. Tissues do not need to be fixed. Frozen sections are directly mounted onto microscope slides.

CryoJane

CryoJane

Components/Accessories:

  • Main unit mounted on and inside cryostat

Capacity: Bone samples and other tissues that are difficult to mount as frozen sections are processed with the CryoJane. The unit provides a permanent adhesive mounting onto slides.

Thermo Shandon Hyperclean Station

Thermo Shandon Hyperclean Station

Components/Accessories:

  • Main Unit
  • Air Filtration Unit

Capacity: Slide mounted specimens can be cover slipped and preserved in mounting medium at this station that provides -air filtration to remove fumes. Small batches of slide mounted specimens can be stained at this station.

Zeiss Axioskop Microscope

Zeiss Axioskop Microscope

Components/Accessories:

  • Microscope
  • Camera
  • Computer

Capacity: Microscope slides are examined and photographed at this station. Photomicrographs are saved onto CDs for permanent storage and publication.

Chargebacks

Access to lab resources/services is contingent upon users’ acceptance of lab operational procedures described under Lab Policies and agreement to pay charges incurred. Fees listed below are in effect starting 11/01/2013.

Slides (after 100 hours)$25/hour
$2,500/year
$0.50/slide$30/hour
$3,000/year
$.50/slideMicrom HM525 cryostat usage
(bring your own blade, book via lab online scheduler listed below)Slides (after 100 hours)$25/hour
$2,500/year
$1.00/slide$30/hour
$3,000/year
$1.00/slide

Service School of Pharmacy Other USC Users
Processing & embedding $5/cassette $6/cassette
Processing & embedding (RUSH) $7.50/cassette $9/cassette
Paraffin sectioning 1st slide $2/slide $2.40/slide
Paraffin sectioning additional slide $1.00/slide $1.20/slide
Frozen sectioning 1st slide $8/slide $9.60/slide
Frozen sectioning additional slide $2/slide $2.40/slide
H&E staining $1.00/slide $1.20/slide
Microm HM310 rotary microtome usage (book via lab online scheduler listed below)
Slide folder (25 slides) $7/Folder $8/Folder
Slide folder (100 slides) $10/Folder $12/Folder
Consultation & Training Free $20/hour

Service Order Forms:

USC School of Pharmacy Order Forms
USC Non-Pharmacy Order Form
Please submit the order form along with tissue samples to Dr. Lina He at PSC402B.

Scheduling

Users are required to utilize CORES to schedule instruments (http://cores-usc.mis.vanderbilt.edu). For users outside of USC, please contact one of the core co-directors (Dr. Jennica Zaro, or Dr. Liana Asatryan, ) to reserve an instrument.

Reservations including changes or cancellations need to be made at least 24 hours in advance. In general, no walk-ins are allowed, however, occasional exceptions may be granted by the lab manager.

Accounts will be billed directly based on the CORES scheduler. With this in mind, users are encouraged to make time estimates as accurate as possible when scheduling. Sufficient time should be made to complete the planned experiment without encroaching on the time slots of other users. A no-show without cancellation will be charged for the time scheduled. New users or users who need technical assistance should indicate so on the reservation form and contact the student tech-in-charge (listed on the reservation page) or the lab manager to make an arrangement for assistance.

Lab Policies

In addition to the general laboratory rules set by USC, users are asked to observe the following procedures that govern the operation of the lab and use of lab resources.

  1. The lab operational hours are 9:00 a.m. to 5:00 p.m., Monday through Friday. Access to the lab outside of the operational hours is discouraged, however, in the case of special circumstances, requests for after-hours access can be submitted to the lab manager. Use of the lab is limited to those resources for which a reservation has been made.
  2. Use of lab resources must be scheduled at least 24 hours in advance using the online scheduler; walk-ins are discouraged although occasional exceptions may be granted by the lab manager.
  3. It is the user’s responsibility to use lab resources in a responsible manner. Users need to contact the lab manager if technical assistance is needed. Damage from misuse will result in charges for repair costs.
  4. Dr. Lina He is in charge of the daily use of the lab. Dr. He trains and directs users who wish to use the lab to conduct their own work. Dr. He also schedules weekend use of the lab by experienced investigators.
  5. Lab support staff must follow the same lab operational procedures and the chargeback policy that apply to all users.

Contact Us

Lab Locations:

USC Health Sciences Campus
USC School of Pharmacy
1985 Zonal Avenue
PSC building (please see individual instruments for lab number)
Los Angeles, CA 90033

Liana Asatryan, PhD
Research Assistant Professor
Co-Director, Translational Research Laboratory
PSC 400
Tel: (323) 442-1554
E-mail:

Jennica Zaro, PhD
Research Assistant Professor
Co-Director, Translational Research Laboratory
PSC 406
Tel: (323) 442-1933
E-mail: