Jianming  Xie

Jianming Xie, PhD

Assistant Professor

Pharmacology and Pharmaceutical SciencesCurriculum Vitae

Research Topics

  1. Chemical Immunology
  2. Protein Chemistry
  3. T Cell Immunology
  4. Immune Monitoring Technology
  5. CAR-T Cell Immunotherapy
  6. Cancer, HIV and Autoimmune Diseases

Contact Information

  • jianminx@usc.edu
  • USC School of Pharmacy
    1985 Zonal Ave PSC 304BA
    Los Angeles CA 90089-9121
  • PSC 304BA
  • 323-442-2341
  • PSC 304A
  • 323-442-0303
  • 323-224-7473

Education

Postdoc, HHMI and Stanford University, 2006-2014
Ph.D., The Scripps Research Institute, 2001-2006
M.S., Shanghai Institute of Organic Chemistry, 1997-2000
B.S., Fudan University, 1992-1997

Jianming Xie

Research Interest

The Xie Lab develops and applies novel Chemical Biology and Protein Engineering tools for understanding and enhancing the function of the immune system to combat cancer, HIV, and autoimmune diseases. Specifically, we are interested in further expanding the scope of a powerful technique that Dr. Xie previously helped pioneer for the site-specific incorporation of unnatural amino acids into recombinant proteins. And more importantly, we integrate this unique tool with other advanced technologies (such as fluorescence microscopy, nanotechnology, and cellular engineering) to interrogate the specificity and efficiency of antigen recognition by conventional and/or CAR-engineered T cells. Additionally, we are interested in using unnatural amino acids to facilitate the design of therapeutic proteins. The long-term goal of our research is to use the obtained knowledge to advance the development of next-generation cellular and protein immunotherapeutics against cancer, HIV, and autoimmune diseases.

Biography

Jianming Xie was born and raised in Putian, China. He received the B.S. degree in Chemistry with highest honors from Fudan University, Shanghai, China, in 1997, and the M.S. degree in Organic Chemistry from Shanghai Institute of Organic Chemistry (SIOC), China, in 2000. In his M.S. research in the laboratory of Prof. Yongzheng Hui and Prof. Biao Yu, he completed the synthesis of a complex natural glycoside using a one-pot four-glycosylation method. He later switched his interest to Chemical Biology, and moved to the United States in 2001 to pursue a Ph.D. degree under the guidance of Prof. Peter Schultz at the Scripps Research Institute, La Jolla, California. There, he helped pioneer a novel biosynthetic method to site-specifically incorporate unnatural amino acids into recombinant proteins in E. coli. His work has enabled the design and synthesis of novel protein structures and functions that do not exist in nature. After receiving his Ph.D. degree in 2006, he became interested in Immunology and joined the laboratory of Prof. Mark Davis as a Cancer Research Institute (CRI) Irvington postdoctoral fellow at Stanford University, Stanford, California. His postdoctoral research integrated the method of site-specific protein modification with fluorescence microscopy and flow cytometry for the study of antigen recognition by T cells. This work revealed ligand-dependent transport of T cell receptors to the immunological synapse, and also led to the development of a protein photochemistry approach to isolate rare antigen-specific T cells from patient blood samples. In December 2014, he started his lab in the Department of Pharmacology and Pharmaceutical Sciences at the University of Southern California (USC) School of Pharmacy. His lab combines synthetic peptide chemistry, protein design, cellular engineering, and fluorescence microscopy in order to interrogate, engineer, and enhance the specificity and efficiency of T cell antigen recognition. The long-term goal is to use the obtained knowledge to guide the design of enhanced immunotherapy and vaccines against cancer, infection, and autoimmune diseases.

Selected Projects/Publications

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Xie J, Huppa JB, Newell EW, Huang J, Ebert PJR, Li Q-J, Davis MM.  Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be ‘preferentially’ transported to the cSMAC. Nature Immunology 2012; 13:674-680.

PubMed

Xie J, Wang L, Wu N, Brock A, Spraggon A, Schultz PG. The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination. Nature Biotechnology 2004; 22:1297-1301.

PubMed

Ueda H; Zhou J, Xie J, Davis MM. Distinct roles of cytoskeletal components in immunological synapse formation and directed secretion. J. Immunol. 2015; 195:4117-25.

PubMed

Kattah NH, Newell EW, Jarrell JA, Chu AD, Xie J, Kattah MG, Goldberger O, Ye J, Chakravarty EF, Davis MM, Utz PJ. Tetramers reveal IL-17 secreting CD4+ T cells that are specific for U1-70 in lupus and mixed connective tissue disease. Proc. Natl. Acad. Sci., 2015; 112: 3044-9. 

PubMed

Xie J, Tato CM, Davis MM. How the immune system talks to itself: The varied role of synapses. Immunological Reviews 2013; 251:65-79.

PubMed

Huang J, Brameshuber M, Zeng X, Xie J, Li Q-J, Chien Y-H, Valitutti S, Davis MM. A single peptide–major histocompatibility complex ligand triggers digital cytokine secretion in CD4+ T cells. Immunity 2013; 39:846-857.

PubMed

Guy CS; Vignali KM, Temirov J, Bettini ML, Overacre AE, Smeltzer M, Zhang H, Huppa JB, Tsai YH, Lobry C, Xie J, Dempsey PJ, Crawford HC, Aifantis I, Davis MM, Vignali DA. Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nature Immunology 2013; 14:262-270.

PubMed

Ebert PJR, Jiang S, Xie J, Li Q-J, Davis MM. An endogenous positively selecting peptide enhances mature T cell responses and becomes an autoantigen in the absence of microRNA miR-181a. Nature Immunology 2009; 10:1162-1170.

PubMed

Kao SC, Wu H, Xie J, Chang CP, Ranish JA, Graef IA, Crabtree GR. Calcineurin/NFAT signaling is required for neuregulin-regulated Schwann cell differentiation. Science 2009; 323:651-654.

PubMed

Xie J, Liu W, Schultz PG. A genetically encoded bidentate, metal-chelating amino acid in E. coli. Angew Chem. Int. Ed. 2007; 46:9239-9242.

PubMed

Xie J, Supekova L, Schultz PG. A genetically encoded metabolically stable analogue of phosphotyrosine in E. coli. ACS Chem. Biol. 2007; 2:474-478.

PubMed

Seyedsayamdost MR, Xie J, Chan CT, Schultz PG, Stubbe J. Site-specific insertion of 3-aminotyrosine into subunit alpha2 of E. coli ribonucleotide reductase: direct evidence for involvement of Y730 and Y731 in radical propagation. J. Am. Chem. Soc., 2007; 129:15060-15071.

PubMed

Xie J, Schultz PG. A chemical tool for proteins – an expanded genetic code. Nature Reviews Mol. Cell. Biol. 2006; 7:775-782.

PubMed

Schultz KC, Supekova L, Ryu Y, Xie J, Perema R, Schultz PG. A genetically encoded infrared probe. J. Am. Chem. Soc., 2006; 128:13984-13985.

PubMed

Wang J, Xie J, Schultz PG. A genetically encoded fluorescent amino acid. J. Am. Chem. Soc., 2006; 128:8738-8739.

PubMed

Bose M, Groff D, Xie J, Brustad E, Schultz PG. The incorporation of a photoisomerizable amino acid into proteins in E. coli. J. Am. Chem. Soc., 2006; 128:388-389. PubMed

Deiters A, Groff D, Ryu Y, Xie J, Schultz PG.  A genetically encoded photocaged tyrosine. Angew Chem. Int. Ed. 2006; 45:2728-2731. 

PubMed

Zeng H, Xie J, Schultz PG. Genetic introduction of a diketone-containing amino acid into proteins. Bioorg. Med. Chem. Letters 2006; 16:5356-5359.

PubMed

Wang L, Xie J, Schultz PG. Expanding the genetic code. Annual Review Biophys. Biomol. Struct. 2006; 35:225-249. 

PubMed

Xie J, Schultz PG. Adding amino acids to the genetic repertoire. Curr. Opin. Chem. Biol, 2005; 9:548-554.

PubMed

Xie J, Schultz PG. An expanding genetic code. Methods 2005; 36:227-238.

PubMed

Wang L, Xie J, Deniz AA, Schultz PG. Unnatural amino acid mutagenesis of green fluorescent protein. J. Org. Chem., 2003; 68:174-176.  

PubMed